Woolworths Kids First is launching a Halloween fundraising event across its UK stores on 25 and 26 October for Children's Hospices UK.
It is the first time the chain has agreed to work with a charity on a single cause.
A regional press campaign will kick off two weeks before the event. Mums and families will be targeted through local papers and radio. The comms work will be handled by the charities' in-house teams.
A spokesperson said that while many of the Woolworths stores exceeded their fundraising expectations, it was hoped this fundraising event would encourage less active employees to get more involved.
'A number of stores support their local hospice already,' said Woolworths MD and Kids First trustees chairman Tony Page. 'It made sense to partner with this national charity to produce a professional, targeted and supported fundraising campaign.'