The government wants to open a single control room for Leicestershire, Nottinghamshire, Northamptonshire, Derbyshire and Lincolnshire as part of plans to replace 50 control rooms across the country with nine.
But Fire Brigade Union (FBU) members have expressed concern over the proposed East Midlands centre's technical capacity, safety, cost and management and are lobbying against the decision at a regional management board meeting this week.
The move is part of a wider FBU campaign opposing Westminster's proposals to open nine regional control rooms.
It wants to maintain local control rooms to keep local accountability and has accused the Office of the Deputy Prime Minister (ODPM) of trying to nationalise the fire control service.
The FBU is gearing up its own efforts to fight the plans in the run-up to a closed ODPM meeting of fire authority chairs on 18 March.
A spokesman said: 'We are presenting our arguments to fire authority councillors, to the chairs of the fire authorities and to regional management board meetings.
'We are also writing to all MPs and especially targeting those who sit on the ODPM's select committee and public accounts committee,' he added.