The awards were set up by UK lobbying firm Westminster Public Affairs.
More than 2,000 people voted to decide the winner. The top-placed Conservative MP in this year's public vote for the Parliamentary Tweeter of the Year was Ed Vaizey MP, finishing in fourth place. Top Liberal Democrat was David Ward MP in fifth and Tom Watson was Labour’s highest placed MP, taking sixth spot.
Wishart, who is MP for Perth and North Perthsire, has more than 13,000 followers on the social networking site. He said: "Social media is increasingly important to get our messages across and is increasingly the place where we all meet to discuss the issues and exchange views. Twitter allows us to have conversations with our constituents, promote the issues that are important to us and it is also just great fun. Most MPs are now on Twitter and it will only continue to grow in importance and influence as the general election approaches."
The judging panel for this year's awards included Michael Crick, political correspondent at Channel 4 News; David Singleton, editor of Public Affairs News; Nick Tyrone, associate director of CentreForum and Olly Kendall, managing director of Westminster Public Affairs.
Kendall said: "It is arguably telling that the top three in the Parliamentary Tweeter of the Year category voted for by the public are not from Westminster’s three biggest parties. It suggests that Twitter can be an effective tool for MPs from the other parties, giving them a platform that the traditional media do not always provide them with."
There were nine other categories: winners included Stella Creasy MP, who won ‘Best Constituency Tweeter’ and Andrew Percy MP who won ‘Funniest Tweet’ with: "My reading for church next Sun has just been delivered. Apparently, reading from my newsletter isn't appropriate so we've gone with the Bble", while Labour leader Ed Miliband MP scooped the ‘Selfie of the year’ award for his shot with Joey Essex and Oscar-winning actor Emma Thompson.