The interactive glasses, which include a camera and a microphone, can record video and browse the web, displaying information through a small screen in front of the wearer’s eye.
To date, only a few thousand people have got their hands on Google Glass but a widespread roll-out is expected later this year.
The etiquette guide has been published on the Google website for members of its "Explorer" programme, the first wearers of the prototype glasses, which cost just under £1,000.
While light-hearted in tone, the etiquette guide makes some serious points, like warning users to ask people’s permission before recording them and warning against looking at the screen for too long.
Don’ts include trying to read "War and Peace on Glass" as "things like that are better done on bigger screens" and wearing Glass while playing high-impact sports.
Google executive chairman Eric Schmidt has previously voiced privacy concerns over the glasses. He told the BBC: "It's obviously not appropriate to wear these glasses in situations where recording is not correct. And, indeed, you have this problem already with phones."